Catabolism of circulating enzymes: plasma clearance, endocytosis, and breakdown of lactate dehydrogenase-1 in rabbits.
نویسندگان
چکیده
منابع مشابه
Catabolism of circulating enzymes: plasma clearance, endocytosis, and breakdown of lactate dehydrogenase-1 in rabbits.
Lactate dehydrogenase-1 (EC 1.1.1.27), intravenously injected into rabbits, was cleared with first-order kinetics (half-life 27 min), until at least 80% of the injected activity had disappeared from plasma. Radioactivity from injected 125I-labeled enzyme disappeared at this same rate. Trichloroacetic-acid-soluble breakdown products started to appear in the circulation shortly after injection of...
متن کاملCatabolism of plasma enzymes, as studied with 125I-labeled lactate dehydrogenase-1 in the rabbit.
Circulating enzymes may be inactivated in the plasma and the inactive breakdown products may be hydrolyzed in the lumen of the small intestine. Evidence for this mechanism was based upon previous studies with 125I-labeled lactate dehydrogenase-5, and here similar studies with radioiodinated lactate dehydrogenase-1 are reported, to determine whether this isoenzyme is similarly catabolized. The p...
متن کاملReceptor-mediated Endocytosis of Lactate Dehydrogenase M, by Liver Macrophages: a Mechanism for Elimination of Enzymes from Plasma
We have previously shown that the rapid clearance of intravenously injected lactate dehydrogenase M4 from plasma is mainly due to endocytosis by macrophages in liver, spleen, and bone marrow. We have now studied endocytosis of lactate dehydrogenase M4 in detail, using freshly isolated rat liver macrophages (Kupffer cells) in vitro. l2”1-lactate dehydrogenase M, rapidly accumulated in the cells ...
متن کاملEndocytosis and breakdown of 125I-labelled lactate dehydrogenase isoenzyme M4 by rat liver and spleen in vivo.
1. Porcine lactate dehydrogenase isoenzyme M4 was labelled with 125I and injected intravenously into rats. Enzyme activity and radioactivity in plasma were cleared in an identical way with a half-life of about 30 min. This half-life was the same as that of unlabelled enzyme. 2. Uptake of label by liver and spleen was determined. Radioactivity in these tissues increased up to about 13 min after ...
متن کاملfaculty of psychology and social sciences group of anthropology master thesis in major of anthropology
چکیده پایان نامه (شامل خلاصه، اهداف، روش های اجرا و نتایج به دست آمده): کار جمع آوری گو یش های محلی در سال های اخیر شتاب امیدوار کننده ای به خود گرفته است. شاید از بارزترین اهداف جمع آوری گویش های مختلف، ثبت و ضبط آن، جلوگیری از نابودی و مهمتر از همه حل مشکلات دستوری زبان رسمی باشد. دقت در فرآیند های زبانی گویش های محلی نوع ارتباط مردم نواحی مختلف با پیرامون نشان را به ما نشان خواهد داد. از س...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1988
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/34.12.2475